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70 PI-MAX
®
4 System Manual Issue 4
• Post-Processing
If you prefer to acquire and preserve the raw image data, make sure that
“Background” is not active on the
Acquisition —> Experiment Setup… —> Data
Corrections
tab {Apply Background Subtraction box is not checked}. Then,
acquire the image, save the raw image data to disk. In WinX you would then use the
Image (or Spectra) Math function (accessed from the
Process menu) to subtract a
background data file from the raw image data: the resulting data can then be saved
to disk in a separate file. In LightField you would open the data in the
Data
Workspace
, select Background… from the Processes drop-down list, choose the
appropriate background file, click on
Preview, and then click on OK. You can now
click on the
Save button, edit the filename if desired, and save the data to disk.
5.8 Readout of the Array
At the end of the exposure, the charge from the pixels is shifted to the shift register,
amplified, and digitized. Depending on the experiment design, binning may also occur as
part of the readout operation.
• For the PI-MAX4:1024i camera and WinX, dual port readout mode is selected
whenever full image readout is selected or when the region of interest (ROI) is
defined to be horizontally symmetrical and centered. Single port readout is
activated when the ROI is not horizontally symmetrical and centered. The same
rules apply whether or not binning is utilized. Readout in single port mode may be
slower than dual port mode readout. The port used for single port mode is dictated
by the CCD design.
• For the PI-MAX4:1024i camera and LightField, when 2 port readout is selected,
you can acquire either a full image or a region of interest (ROI) defined to be
horizontally symmetrical and centered. If the ROI is not horizontally symmetrical
and centered, single port readout must be selected. The same rules apply whether or
not binning is utilized. Readout in single port mode may be slower than dual port
mode readout. The port used for single port mode is dictated by the CCD design.
• For the PI-MAX:1024x256, the signal is read out of a single port.