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188 PI-MAX
®
4 System Manual Issue 4
13.3.3.2 Sequential
Perform the following procedure to configure Sequential Gating Mode:
1. The first requirement is that the PI-MAX4: 1024i-RF camera be aligned and focused on
the area of interest in the experiment. This is best accomplished while the camera is
operating in Full Frame readout mode. Verify that the
Phosphor Decay Delay is
appropriate to the phosphor used by your camera: the phosphor decay delay time
entered in LightField can be viewed or changed after clicking on the
Advanced button
on the
Common Acquisition Settings expander. The procedure for initial focus is
described in Chapter 4, First Light.
NOTE:
The Phosphor Decay Delay setting is used to tell LightField
how long to wait after the gate pulse to shift the image. If there
is some residual image from the first frame in the second
frame, simply increase the Phosphor Decay Delay setting to
allow more time for the phosphor emission to decay before
shifting the image.
2. After the alignment and focus, the PI-MAX: 1024i-RF system needs to be put into RF
modulation mode. On the
Common Acquisitions Settings expander, select Use RF
Modulation
.
3. Enter 2 or more in the Number of Frames field. The range is 2 to 1,023.
4. To track the modulation phase, click in the Modulation Tracking check box.
5. On the Trigger expander, select Internal or External triggering.
• For External triggering, make sure the trigger characteristics on the Trigger
expander match the active trigger edge, etc. of the trigger pulse that will be used.
• For Internal triggering, set the Internal Trigger Frequency on the
SuperSYNCHRO Timing expander.
6. Open the SuperSYNCHRO Timing expander (at the bottom of the window.) See
Figure 13-3.
Figure 13-3: SuperSYNCHRO Timing Expander: Sequential Gating Mode
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