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Optimizing Performance
Operational Hints for the Multi Channel Gradient Valve (MCGV)
Operational Hints for the Multi Channel
Gradient Valve (MCGV)
In a mixture of salt solutions and organic solvent the salt solution might be
well dissolved in the organic solvent without showing precipitations.
However in the mixing point of the gradient valve, at the boundary between
the two solvents, micro precipitation is possible. Gravity forces the salt
particles to fall down. Normally the A channel of the valve is used for the
aqueous/salt solution and the B channel of the pump is used for the organic
solvent. If used in this configuration the salt will fall back into the salt
solution and will be dissolved. When using the pump in a different
configuration (e.g., D - salt solution, A -organic solvent) the salt can fall into
the port of the organic solvent and may lead to performance problems.
NOTE When using salt solutions and organic solvents in the Agilent 1100 Quaternary
Pump it is recommended to connect the salt solution to one of the bottom
ports and the organic solvent to one of the upper gradient valve ports. It is best
to have the organic channel directly above the salt solution channel.
Regular flushing with water of all MCGV channels is recommended to remove
all possible salt deposits in the valve ports.